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http://krishi.icar.gov.in/jspui/handle/123456789/5145| Title: | Affinity purification and partial characterization of serum immunoglobulin of Clarias gariepinus |
| Other Titles: | Not Available |
| Authors: | Gaurav Rathore T Raja Swaminathan, Neeraj Sood B N Mishra D Kapoor |
| Published/ Complete Date: | 2006-12 |
| Project Code: | Not Available |
| Keywords: | Chromatography Clarias gariepinus Fish Immunoglobuli |
| Publisher: | CSIR |
| Citation: | Rathore, G., Swaminathan, T. R., Sood, N., Mishra, B. N., & Kapoor, D. (2006). Affinity purification and partial characterization of IgM-like immunoglobulins of African catfish, Clarias gariepinus (Burchell, 1822). |
| Series/Report no.: | Not Available; |
| Abstract/Description: | gM like macroglobulin from bovine serum albumin (BSA)-immunized African catfish C. gariepinus was purified by affinity chromatography and partially characterized. The molecular weight of this macroglobulin was 840 kDa, as estimated by gel filtration chromatography. Purified macroglobulin was analyzed using SDS-PAGE under reducing and non-reducing conditions. The molecular weight (MW) of heavy and light chain was 74.8 kDa and 27.2 kDa respectively, in presence of a reducing agent. In non-reducing SDS-PAGE, a single high MW band was observed representing tetrameric form. |
| Description: | Not Available |
| ISSN: | 975-1009 (Online); 0019-5189 (Print) |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Experimental Biology |
| NAAS Rating: | 6.78 |
| Volume No.: | 44: |
| Page Number: | 1018-1021 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://hdl.handle.net/123456789/6669 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5145 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 44(12) 1018-1021.pdf | 126.47 kB | Adobe PDF | View/Open |
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