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http://krishi.icar.gov.in/jspui/handle/123456789/5186| Title: | Primary cell culture from fish gills and kidney using fish serum |
| Other Titles: | Not Available |
| Authors: | Gaurav Rathore Neeraj Sood Raja Swaminathan |
| Published/ Complete Date: | 2001-09 |
| Project Code: | Not Available |
| Keywords: | cell culture gills and kidney fish serum |
| Publisher: | NISCAIR-CSIR, INDIA |
| Citation: | Rathore, Gaurav, Neeraj Sood, and Raja Swaminathan. "Primary cell culture from fish gills and kidney using fish serum." (2001). |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A novel, cost effective and time saving technique for primary cell culture from gills and kidney using fish serum has been developed. Single cell suspension of gills and kidney of Clarias gariepinus was obtained by trypsinization. Minimum essential medium supplemented with 10% fish serum in place of 10% foetal calf serum and 20% fish muscle extract, yielded confluent monolayer on 6th and 8th day in gill and kidney tissue respectively at 28°C. Fish serum can be successfully used as media supplement for cultivation and maintenance of primary cell culture from fishes. |
| Description: | Not Available |
| ISSN: | 0975-1009 (Online); 0019-5189 (Print) |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Experimental Biology |
| NAAS Rating: | 6.78 |
| Volume No.: | 39(9) |
| Page Number: | 936-938 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://hdl.handle.net/123456789/24033 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/5186 |
| Appears in Collections: | FS-NBFGR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJEB 39(9) 936-938.pdf | 883.14 kB | Adobe PDF | View/Open |
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