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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/53267
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Venkataravanappa, V., Priti Sonavane, Lakshminarayana Reddy, C. N., Reddy, M. K., | en_US |
dc.date.accessioned | 2021-08-05T08:33:13Z | - |
dc.date.available | 2021-08-05T08:33:13Z | - |
dc.date.issued | 2020-07-01 | - |
dc.identifier.citation | Venkataravanappa, V., Priti Sonavane, Lakshminarayana Reddy, C. N., Reddy, M. K., 2020, Characterization of novel French bean leaf curl virus and beta satellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.), Journal of General Plant Pathology, https://doi.org/10.1007/s10327-020-00979-3 | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/53267 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Leaves from French bean plants with severe leaf curl (ten samples) and from asymptomatic plants from 10 felds (FB1–10) in the Western Ghat region of Karnataka, India were collected. Virus isolate FB01 from French bean was shown to be transmitted by whitefy Bemisia tabaci cryptic species Asia I. DNA was isolated from the samples (ten feld samples and one sample infected with FB01 via whitefy) and subjected to PCR to confrm begomovirus infection using specifc primers. All 11 were positive for the expected 1.2-kb amplicon. The sequence analysis of the 1.2-kb amplicon showed that all isolates shared more than 98% nucleotide identity. Further, the complete genome from isolate FB01 was amplifed and sequenced and shown to share a maximum nucleotide identity (89.3%) with Ageratum yellow vein Sri Lanka virus. As per the ICTV species threshold for begomoviruses, the virus is considered as a new species, with the proposed name French bean leaf curl virus (FbLCV). The samples were subjected to PCR using specifc primers for DNA B, betasatellite and alphasatellite; only betasatellites were amplifed. The detected betasatellite shared maximum nucleotide identity (92.6%) with Synedrella yellow vein betasatellite. Recombination analysis indicated that FbLCV and betasatellite were recombinants. The signifcance of these fndings are discussed. | en_US |
dc.description.sponsorship | Indian Council of Agricultural Research | en_US |
dc.language.iso | English | en_US |
dc.publisher | Journal of General Plant Pathology | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Begomovirus · Recombination · Phylogenetic analysis · Polymerase chain reaction · Betasatellite | en_US |
dc.title | Characterization of novel French bean leaf curl virus and beta satellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.), | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | HORTIIHRCIL2014175 | en_US |
dc.publication.journalname | Journal of General Plant Pathology | en_US |
dc.publication.volumeno | 87 | en_US |
dc.publication.pagenumber | 94-100 | en_US |
dc.publication.divisionUnit | Crop Protection | en_US |
dc.publication.sourceUrl | https://doi.org/10.1007/s10327-020-00979-3 | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Horticultural Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | open access | en_US |
dc.publication.naasrating | 6.97 | en_US |
Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
File | Description | Size | Format | |
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French bean.pdf | 912.55 kB | Adobe PDF | View/Open |
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