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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/53393
Title: | Mining of miRNAs using Next Generation Sequencing (NGS) data generatedfor Okra (Abelmoschus esculentus) |
Other Titles: | Not Available |
Authors: | Rekha Gupta, M. Gayathri , V. Radhika, M. Pichaimuthu and *K.V. Ravishankar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR -Indian Institute of Horticultural Research, Hesaraghatta lake post, Bengaluru - 560089 |
Published/ Complete Date: | 2018 |
Project Code: | Not Available |
Keywords: | microRNA; Next Generation Sequencing; Abelmoschus esculentus; miRNA targets. |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | ABSTRACTMicroRNAs (miRNAs) are small, highly conserved non-coding RNA molecules involved in theregulation of gene expression in eukaryotes. Gene expression involves post-transcriptionalgene regulation by miRNAs. miRNAs are formed from precursor RNA molecules that fold intoa stem loop secondary structure. The mature miRNA is one end of the precursor miRNA,defined by the cut from ‘Drosha’ on either the 5’ or 3’ arm. In this study, we have used abioinformatics approach to identify miRNAs in 3,361 contigs obtained from partial genomesequence data of Abelmoschus esculentus (okra) sequenced by NGS technology. Using C-miiand psRNA Target tools, we identified two miRNAs and their target RNAs for which a regulatorymiRNA binding has been verified. Their targets consisted of transcription factors involved ingrowth and development, gene regulation and metabolism. Phylogenetic analysis of the newlyidentified miRNA family has been done to compare their level of conservation with respect tothe other members of the plant kingdom. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Horticultural Science and Biotechnology (Journal of Horticultural Science, England) |
NAAS Rating: | 7.16 |
Volume No.: | 13(2) |
Page Number: | 137-145 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/53393 |
Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
File | Description | Size | Format | |
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Mining of miRNA'S.pdf | 3.17 MB | Adobe PDF | View/Open |
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