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http://krishi.icar.gov.in/jspui/handle/123456789/55273
Title: | Molecular characterization of Steinernema abbasi from mango orchards using ITS region based markers |
Other Titles: | Not Available |
Authors: | Kumar, H.K., Muthukumar, M., and RM Khan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Sub-tropical Horticulture |
Published/ Complete Date: | 2016-01-05 |
Project Code: | Not Available |
Keywords: | nematode |
Publisher: | The Nematological Society of India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Soil samples were collected from the mango orchards at CISH, Rehmankhera for isolation of EPNs. A population of Steinernema sp. was extracted from soils by Galleria mellonella baiting technique (Bedding and Akhurst, 1975). The samples were purified for Koch postulates and maintained in the BOD incubator at 15 C. For molecular studies, DNA was isolated from fresh infective juveniles (one week old) by using 50 µl of worm lysis buffer (50 mM KCl, 10mm tris, 205 mm MgCl2, 0.45% NP40, 0.45% Tween 20, 0.01% gelatin and 60 µg/ml proteinase K) and homogenizing the nematodes. The suspension was incubated at 95 C for 10 min, 65 C for 1 h, and at-80 C for 2 h and finally stored in-20 C for further use. DNA was checked for its quality and quantified using UV-spectrophotometry. PCR reaction was carried out using the components in the following conditions: 10 µl of 10 X PCR buffer, 15 mM Mgcl2, 2 µl of 5 |
Description: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Nematology |
NAAS Rating: | 5.23 |
Volume No.: | 46(1) |
Page Number: | 75-76 |
Name of the Division/Regional Station: | Division of Crop Improvement and Biotechnology |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/55273 |
Appears in Collections: | HS-CISH-Publication |
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