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http://krishi.icar.gov.in/jspui/handle/123456789/56222
Title: | Micropropagation of guava |
Other Titles: | Not Available |
Authors: | Mishra,M., Chandra,R.,Pati,R. and Bajpai,A. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR_CISH |
Published/ Complete Date: | 2007-01-01 |
Project Code: | Not Available |
Keywords: | shoot bud culture, proliferation, MS media |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A foolproof method of micropropagation through in vitro shoot bud culture has been developed in guava cv. ‘Allahabad Safeda’. The stock plant of guava was maintained at shade net house (75% shade) and pruned severely to encourage new growth. Around 3 cm long shoots were excised and defoliated. The explants were pretreated in solution containing 0.1% Carbendazime and 100 mg/L PVP for 1 hour and then washed with Tween-20 wetting agent. The pretreated explants were further treated with HgCl2 0.1% for 5 minutes aseptically followed by six washing in autoclaved distilled water. The sterilized explants were cultured on MS medium supplemented with BAP (3.0 mg/L) for shoot bud induction and proliferation. The proliferated shoots were then subcultured on MS medium containing 10 mg/L IBA for rhizogenesis. The rooted plants were finally shifted to autoclaved coconut husk fortified with ½ MS salt mixture for acclimatization. Acclimatized plantlets of guava cv. Allahabad Safeda were planted. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Acta Horticulturae |
Volume No.: | Not Available |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/56222 |
Appears in Collections: | HS-CISH-Publication |
Files in This Item:
File | Description | Size | Format | |
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Micropropagation of guava. Acta Hort.pdf | 80.3 kB | Adobe PDF | View/Open |
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