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http://krishi.icar.gov.in/jspui/handle/123456789/59170| Title: | Relative expression of apocarotenoid biosynthetic genes in developing stigmas of Crocus sativus L. |
| Other Titles: | Not Available |
| Authors: | J I MIR N AHMED T A MOKHDOMI A H WAFAI S H WANI S BUKHARI A AMIN R A QADRI |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Central Institute of Temperate Horticulture, Srinagar |
| Published/ Complete Date: | 2013-01-01 |
| Project Code: | Not Available |
| Keywords: | Crocus sativus CsBCH CsGT-2 CsLYC, CsZCD stigma development |
| Publisher: | Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Saffron, the desiccated stigmas of Crocus sativus, is recognized for its attractive color, flavor, and aroma which are due to the accumulation of crocin, picrocrocin, and safranal, respectively. HPLC analysis demonstrated maximum apocarotenoid accumulation during the fully developed scarlet stage of stigma development followed by the orange and yellow stages of stigma development. Reverse Transcription-PCR analysis revealed a concurrent expression pattern of CsZCD and CsLYC genes in a developmental stagespecific manner. However, CsBCH and CsGT2 genes were specifically expressed during the mature, scarlet stage of stigma development. Real-Time PCR analysis showed a sharp increase in gene expression of CsLYC gene during stigma development indicative of its possible regulatory role in apocarotenoid biosynthesis or stigma development. Results suggest that genetic manipulation of this gene can help to improve the quality of stigma in saffron; besides highlighting its potential to monitor stigma development during in vitro experimentation |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Crop Science and Biotechnology |
| Journal Type: | Hybrid (Transformative Journal) |
| Volume No.: | 16(3) |
| Page Number: | 183-188 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.researchgate.net/publication/259641720_Relative_Expression_of_Apocarotenoid_Biosynthetic_Genes_in_Developing_Stigmas_of_Crocus_sativus_L |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/59170 |
| Appears in Collections: | HS-CITH-Publication |
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