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http://krishi.icar.gov.in/jspui/handle/123456789/60785
Title: | Distinction between glycomacropeptide and β-lactoglobulin with ‘stains all’ dye on Tricine SDS-PAGE gels. |
Other Titles: | Not Available |
Authors: | Sharma, N.; Sharma, R.; Rajput, Y.S.; Mann, B. and Gandhi, K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Dairy Research Institute |
Published/ Complete Date: | 2021-03-15 |
Project Code: | Not Available |
Keywords: | Glycomacropeptide, β-Lactoglobulin‘Stains all’ dye, SDS-PAGE, Whey |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Identification of glycomacropeptide (GMP) and β-lactoglobulin (β-lg) present in cheese whey is difficult on SDS-PAGE due to their close proximity during electrophoresis and poor sensitivity of commonly used staining dye ‘coomassie brilliant blue’ (CBB) towards GMP. A simple method has been developed for the detection of GMP and β-lg by staining acrylamide gel after tricine SDS-PAGE using cationic ‘stains all’ dye. After staining and destaining major whey proteins, viz. ɑ-lactalbumin (ɑ-la) and β-lg appear red while GMP stains blue. The method can be used for the identification of these macromolecules in cheese whey and the detection of adulteration of milk with rennet whey. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Food Chemistry |
NAAS Rating: | 12.31 |
Impact Factor: | 7.4 |
Volume No.: | 340 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Dairy Chemistry |
Source, DOI or any other URL: | 10.1016/j.foodchem.2020.127923 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/60785 |
Appears in Collections: | AS-NDRI-Publication |
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