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http://krishi.icar.gov.in/jspui/handle/123456789/62012| Title: | Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.) |
| Other Titles: | Not Available |
| Authors: | A K Lincy A B Remasree B Sasikumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Spices Research |
| Published/ Complete Date: | 2009 |
| Project Code: | Not Available |
| Keywords: | Aerial stem Zingiber histology somatic embryogenesis |
| Publisher: | ICAR- Indian institute of spices research,Kozhikode, Kerala India |
| Citation: | Lincy, Adinkudik & B, Rema shree. (2009). Indirect and direct somatic embryogenesis from aerial stem explants of ginger (Zingiber officinale Rosc.). Acta Botanica Croatica (acta@biol.pmf.hr); Vol.68 No.1.. 68. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Protocols for direct and indirect somatic embryogenesis from aerial stem explants of ginger have been developed. Aerial stem explants of two ginger varieties were cultured on different concentrations of 2,4-D induced callus. An in vitro aerial stem produced hard,nodular and yellowish callus (Type I) and an in planta aerial stem gave rise to soft, sticky callus with pale white color (Type II). The proliferated Type II calli were subject to stress for 40–60 days without subculturing. The desiccated calli produced white friable calli which turned embryogenic and then produced somatic embryos in a medium containing 2mg L–1 benzyl amino purine. The mature, club-shaped somatic embryos were germinated on a medium containing benzyl amino purine and a– naphthalene acetic acid in different concentrations. Type I callus of neither variety turned embryogenic but produced roots in all the cultures. Direct somatic embryogenesis was observed from the in planta aerial stem and leaf base explants with the use of thidiazuron alone or in combination with indole, 3-butyric acid. Histological studies revealed that the somatic embryos in gingerhave a distinct single layered epidermis, scutellum, coleoptile, shoot apex and root apex |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Acta Botanica Croatica |
| NAAS Rating: | 7.05 |
| Volume No.: | 68(1) |
| Page Number: | 93- 103 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.researchgate.net/publication/27219463_Indirect_and_direct_somatic_embryogenesis_from_aerial_stem_explants_of_ginger_Zingiber_officinale_Rosc |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/62012 |
| Appears in Collections: | HS-IISR-Publication |
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