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http://krishi.icar.gov.in/jspui/handle/123456789/62773| Title: | Development and characterization of two cell lines PDF and PDH from Puntius denisonii (Day 1865) |
| Other Titles: | Not Available |
| Authors: | Sigh,M Patiyal,R S Gopalakrishnan,A Yadav,Kamalendra Goswami,Mukunda Lakra,W S |
| ICAR Data Use Licennce: | Not Available |
| Author's Affiliated institute: | Not Available |
| Published/ Complete Date: | 2011 |
| Project Code: | Not Available |
| Keywords: | Puntius denisonii PDH Cell line |
| Publisher: | Springer |
| Citation: | Not Available |
| Series/Report no.: | Not Available |
| Abstract/Description: | The Puntius denisonii colloquially and more popularly referred to as Miss Kerala is a subtropical fish belonging to the genus Puntius (Barb) and family Cyprinidae. Two cell lines PDF and PDH were developed from the caudal fin and heart of P. denisonii, respectively. The cell lines were optimally maintained at 26°C in Leibovitz-15 medium supplemented with 10% fetal bovine serum. A diploid count of 50 chromosomes at passage 50 was observed in both the cell lines. The high growth potential of the cell lines was reflected from the cell doubling time of 28 and 30 h of PDF and PDH cell lines, respectively. The viability of the PDF and PDH cell lines was 70% and 76%, respectively, after 4 mo of storage in liquid nitrogen (−196°C). The origin of the cell lines was confirmed by the amplification of 653 bp fragments of cytochrome oxidase subunit I of mitochondrial DNA genes. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | In Vitro Cellular & Developmental Biology - Animal |
| Volume No.: | 47 |
| Page Number: | 89-94 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.ncbi.nlm.nih.gov/pubmed/21136193 http://eprints.cmfri.org.in/11857/1/AGKN_2011_In Vitro Cellular & Developmental Biology - Animal_Puntius denisonii cell line Lakra.pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/62773 |
| Appears in Collections: | FS-CMFRI-Publication |
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