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http://krishi.icar.gov.in/jspui/handle/123456789/64689
Title: | Construction and screening of metagenomic library derived from soil for β-1, 4-endoglucanase gene |
Other Titles: | Not Available |
Authors: | Pandey, S. Gulati, S., Goyal, E., Singh, S., Kumar, K., Nain, L. and Saxena, A.K |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-NATIONAL BUREAU OF AGRICULTURALLY IMPORTANT MICROORGANISMS KUSHMAUR MAU NATH BHANJAN 275103 |
Published/ Complete Date: | 2016-01-01 |
Project Code: | Not Available |
Keywords: | Metagenomic libraryCellulaseFunction based screeningβ-1, 4-endoglucanasePaper and pulp mill |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | A metagenomic library consisting of 7500 clones was constructed from the soil continuously irrigated with effluents of paper and pulp mill. A cellulase positive clone H-10 was identified by function based screening. A β-1, 4- endoglucanase gene with an open reading frame of 1499 bp was identified from this positive clone. The deduced amino acid sequence analysis revealed that amplified β-1, 4-endoglucanase belongs to GH5 and shares 98% similarity with Bacillus subtilis (EF070194), 95% with Bacillus subtilis IARI-SP-1(KF240847) 96% with B. subtilis (AY183475) and 95% with uncultured bacterium clone (JX567739). Clone H-10 indicated higher CMCase activity of 1 IU/ml on the substrate CMC as compared to rice straw (0.5 IU/ml). The characterization of enzymes revealed its pH optima between 8.5–9 and retained 80% of activity at pH 11. The thermal profile of the enzyme indicated broad range of thermotolerance. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Biocatalysis and Agricultural Biotechnology |
Volume No.: | 5 |
Page Number: | 186-192 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/64689 |
Appears in Collections: | CS-NBAIMO-Publication |
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