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http://krishi.icar.gov.in/jspui/handle/123456789/73078| Title: | Development of a reverse transcription (RT) polymerase chain reaction (PCR) method for the detection of human norovirus in bivalve molluscs. |
| Other Titles: | Not Available |
| Authors: | Lekshmi M, Kumar SH, Rajendran KV, Nayak BB |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
| Published/ Complete Date: | 2021-02-01 |
| Project Code: | Not Available |
| Keywords: | enteric virus, norovirus GII, RT-PCR, seafood, shellfish |
| Publisher: | IWA Publishing |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Noroviruses are significant seafood-borne pathogens, commonly associated with the consumption of filter feeding bivalve molluscs. Here, we report the development of a reverse transcription polymerase chain reaction (RT-PCR) method using primers based on the RNA-dependent RNA polymerase gene of norovirus genogroup II (NoV GII). Samples of bivalves were processed for the concentration of virus and extraction of RNA, followed by reverse transcription PCR. A total of 50 molluscan shellfish samples were analyzed, of which 16 samples yielded positive amplifications of norovirus nucleic acid. The PCR method described here, involving a single set of primers, is useful for rapid screening of shellfish for NoV GII. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Water Science and Technology |
| Journal Type: | International |
| NAAS Rating: | 7.53 |
| Impact Factor: | 1.53 |
| Volume No.: | 83 (5) |
| Page Number: | 1103-1107 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/73078 |
| Appears in Collections: | FS-CIFE-Publication |
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