KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/73863
Title: | Molecular characterization of inhibin-A: Structure and expression analysis in Clarias batrachus. |
Other Titles: | Not Available |
Authors: | Ahmad I, Dhanashree D, Jagtap DD, Kumar CS, Balasinor NH, Rani B, Agarwal D, Saharan N* |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR: Central Institute of Fisheries Education |
Published/ Complete Date: | 2018-02-01 |
Project Code: | Not Available |
Keywords: | Asian catfish Inhibin-A Protein-protein docking Glycosylation Tissue distribution |
Publisher: | ELSEVIER |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The inhibins are disulphide-linked heterodimeric glycoproteins that belong to the TGFb superfamily. Inhibins have been well studied in mammals but the information about their structure and function is very limited in lower vertebrates. The aim of the present study was to characterize inhibin-A and to understand its receptor binding interaction, and to evaluate its biological function in Clarias batrachus. Structure prediction of inhibin-A revealed two glycosylation sites on inhibin-a (Asp262 and Asn334). Docking of inhibin-A with its receptor; betaglycan and Act RIIA showed that residues Ser321, Gly324 and Leu325 of inhibin-a are involved in high affinity binding with betaglycan while inhibin-bA bound to Act RIIA by forming hydrogen bonds. The mRNA transcript analysis of various tissues indicated the presence of higher to moderate expression of inhibin-a and inhibin-bA in the gonads and the extra- gonadal tissues. Further, stage specific expression showed decreased levels of inhibin-a in the gonads during the annual reproductive cycles. Inhibin-bA, activin-bB and Act RIIA increased in the brain during spawning while FSHr increased in the gonads during the preparatory phase. Our study provides molec- ular, structural and functional insights of inhibin-A for the first time in C. batrachus. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | General and Comparative Endocrinology |
Journal Type: | International |
NAAS Rating: | 8.82 |
Impact Factor: | 2.822 |
Volume No.: | 261 |
Page Number: | 104-14 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/73863 |
Appears in Collections: | FS-CIFE-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.