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http://krishi.icar.gov.in/jspui/handle/123456789/7450
Title: | Electrophoresis-staining apparatus for DNA agarose gels with solution exchange and image acquisition |
Other Titles: | Not Available |
Authors: | Praveen Maurye, Arpita Basu, Jayanta Kumar Biswas, Tapas Kumar Bandyopadhyay |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Central Inland Fisheries Research Institute (I.C.A.R.), Barrackpore, Kolkata, West Bengal 700 120. India. |
Published/ Complete Date: | 2017-01-02 |
Project Code: | Not Available |
Keywords: | Agarose gel electrophoresis, image acquisition, nucleic acids, staining |
Publisher: | Taylor & Francis |
Citation: | Electrophoresis-staining apparatus for DNA agarose gels with solution exchange and image acquisition P Maurye, A Basu, JK Biswas, TK Bandyopadhyay - Instrumentation Science & Technology, 2017 |
Series/Report no.: | Not Available; |
Abstract/Description: | Agarose gel electrophoresis is routinely used for the separation of nucleic acids. Gels are developed, stained, and visualized using dedicated equipment and reagents. Manufacturers have developed instrumentation with advanced features that provide good safety and user-friendly operation. However, the process of size fractionation of nucleic acids by horizontal gel electrophoresis by dye staining may be cumbersome and unsafe due to many steps and harmful chemicals. Here is reported a safe, inexpensive, time-saving, and comprehensive apparatus for gel electrophoresis, staining, and imaging. This newly modified apparatus has simple operation and uses existing equipment and off-the-shelf components for easy construction in the laboratory. The apparatus has been shown to perform agarose horizontal gel electrophoresis and associated techniques with ease and simplicity. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Instrumentation Science & Technology |
NAAS Rating: | 7.42 |
Volume No.: | 45 |
Page Number: | 49-61 |
Name of the Division/Regional Station: | Fishery Resource and Environment Management Division |
Source, DOI or any other URL: | https://doi.org/10.1080/10739149.2016.1206926 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7450 |
Appears in Collections: | FS-CIFRI-Publication |
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