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http://krishi.icar.gov.in/jspui/handle/123456789/75076| Title: | Amplified ribosomal DNA restriction analysis and Random amplified polymorphic DNA analysis; useful genetic markers for mycoparasite diagnostics in edible mushrooms |
| Other Titles: | Not Available |
| Authors: | VP Sharma, Shwet Kamal and Satish Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Directorate of Mushroom Research, Solan |
| Published/ Complete Date: | 2014-01-01 |
| Project Code: | Not Available |
| Keywords: | Mushroom, fungal diseases, mycoparasites, rDNA, ARDRA, RAPD, diagnostics |
| Publisher: | Mushroom Society of India |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Three arbitrary decamer RAPD primers amplified unique scorable DNA fragments and exhibited variations among different mycoparasites of edible mushrooms. Tag 1 restriction enzyme produced unique Amplified Ribosomal DNA Restriction Analysis (ARDRA) profiles in four important mycoparasites, namely, Hypomyces perniciosus (200 bp), Cladobotryum asterophorum (280bp), Cladobotryum mmycophilum (300 bp), Papulospora byssina (400 bp). Whereas, Msp 1 restriction enzyme yielded unique prfiles for identification of Charactomium globosum (440 bp), Trichoderma harzianum (380 bp), Trichoderma longibrachiatum (300 bp), and Sepedonium chryospermum (350 bp), that can be used as genetic markers for quick detection of these mycoparasites without resorting each time to DNA sequencing. |
| Description: | Not Available |
| ISSN: | 0972-4885 |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Mushroom Research |
| Journal Type: | International |
| NAAS Rating: | 4.34 |
| Volume No.: | 23(2) |
| Page Number: | 191-197 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/75076 |
| Appears in Collections: | HS-DMR-Publication |
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