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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77122
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Rathor PK, Rather MA, Gireesh-Babu P, Kumar K, Purayil SBP, Sharma R* | en_US |
dc.date.accessioned | 2023-05-12T03:59:10Z | - |
dc.date.available | 2023-05-12T03:59:10Z | - |
dc.date.issued | 2017-06-21 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/77122 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Steroidogenic acute regulatory protein (StAR) is responsible for the relocation of cholesterol across mito- chondrial membrane in vertebrates and is, therefore, a key factor in regulating the rate and timing of steroidogenesis. In the present study, we developed chitosan nanoparticle (CNP) conjugated StAR gene construct (CNP-pcDNA4-StAR) in a eukaryotic expression vector, pcDNA4/HisMax A. CNPs of 135.4 nm diameter, 26.7 mV zeta potential and 0.381 polydispersity index were used for conjugation. The loading efficiency (LE) of pcDNA4-StAR construct with CNPs was found to be 86%. After the 24 h of intramuscular injection, the CNP-pcDNA4-StAR plasmid could be detected from testis, brain, kidney and muscle tissues of Clarias batrachus. The transcript levels of important reproductive genes viz. cyp11a1, cyp17a1, 3 -hsd, 17 -hsd and cyp19a1 in CNP-pcDNA4-StAR treated group were initially low up to 24 h, but significantly increased subsequently up to 120 h. In naked pcDNA4-StAR treated group, the mRNA level of 3 -hsd, 17 -hsd and cyp19a1 increased initially up to 24 h, while cyp11a1 and cyp17a1 increased up to 48 h and then started declining. Similar results were obtained for 11-Ketotestosterone and 17 -estradiol. The results indicate relatively long lasting effects of nano-conjugated construct compared to the construct alone. Furthermore, the histopathology of gonads and liver authenticates its possible role in the gonadal development in fish without any adverse effect. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | ELSEVIER | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | StAR gene Expression vector DNA construct Chitosan nanoparticles Nanodelivery | en_US |
dc.title | Steroidogenic acute regulatory protein (StAR) gene expression construct: Development, nanodelivery and effect on reproduction in air-breathing catfish, Clarias batrachus | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | International Journal of Biological Macromolecules | en_US |
dc.publication.volumeno | 104 | en_US |
dc.publication.pagenumber | 1082-1090 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR: Central Institute of Fisheries Education | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | International | en_US |
dc.publication.naasrating | 9.67 | en_US |
dc.publication.impactfactor | 6.953 | en_US |
Appears in Collections: | FS-CIFE-Publication |
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