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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/77318
Title: | Occurrence, pathogroup distribution and virulence genotypes of Escherichia coli from fresh seafood. |
Other Titles: | Not Available |
Authors: | Not Available |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Prakasan S, Lekshmi M, Ammini P, Balange AK, Nayak BB, Kumar SH* |
Published/ Complete Date: | 2021-11-12 |
Project Code: | Not Available |
Keywords: | Seafood Escherichia coli Pathogroup Fecal contamination Risk assessment Serotype |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Seafood contamination with pathogenic Escherichia coli is a significant public health threat in developing countries. In this study, the prevalence of different pathogroups of E. coli was investigated in fresh seafood. A total of 78 samples comprising of finfish (41) and shellfish (37) were analyzed using multiple selective- enrichment-isolation protocols. From all samples, 639 E. coli were isolated and tested for the presence of pathogroup-specific virulence-associated genes by polymerase chain reaction (PCR). Based on the distribution of virulence genes, 118 isolates belonged to enterohemorrhagic E. coli (EHEC) group, 25 to enteropathogenic E. coli (EPEC), 20 to enterotoxigenic E. coli (ETEC), 16 to enteroaggregative E. coli (EAEC) and 1 to enteroinvasive E. coli (EIEC). Fifty-five samples of seafood, 29 finfish and 26 shellfish, were found contaminated with pathogenic E. coli. Among different enrichment broth-selective agar combinations used for the recovery of pathogenic E. coli from seafood, enrichment in Tryptone Phosphate (TP) broth followed by isolation on sorbitol MacConkey (SMAC) agar performed superior to others. The study highlights the importance of using selective isolation and genotyping protocols to identify human pathogenic E. coli in seafood in regions with extensive anthropogenic contamination. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Food Control |
NAAS Rating: | 11.55 |
Impact Factor: | 5.55 |
Volume No.: | 133 |
Page Number: | Not Available |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77318 |
Appears in Collections: | FS-CIFE-Publication |
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