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http://krishi.icar.gov.in/jspui/handle/123456789/79092| Title: | Effect of betaine and raffinose on post-thaw semen parameters and fertility in black pigmented Kadaknath chicken |
| Other Titles: | Not Available |
| Authors: | Pranay B., Swathi B., Shanmugam M |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Directorate of Poultry Research |
| Published/ Complete Date: | 2022-09-01 |
| Project Code: | Not Available |
| Keywords: | betaine; chicken; cryopreservation; fertility; raffinose; Kadaknath breed |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | BACKGROUND: Kadaknath is an important indigenous chicken with black pigmentation and cryopreserved semen reputably had low fertility. OBJECTIVE: The aim of this study was to evaluate the effects of betaine and raffinose in semen extenders on post thaw semen parameters and fertility. MATERIALS AND METHODS: Semen was cryopreserved in 4% dimethyl sulfoxide (DMSO) with betaine supplemented at 0.1, 0.2 and 0.4 M or raffinose supplemented at 1, 5 and 10 mM. Post thaw semen parameters and fertility were evaluated. RESULTS: Betaine at higher concentrations significantly (p < 0.05) inhibited the post thaw sperm motility, live sperm and MTT dye reduction and a declining trend in the fertility with increasing betaine. Inclusion of raffinose had no effect on the post thaw in vitro semen parameters, however, the fertility was significantly (p < 0.05) higher in the 10 mM raffinose supplemented group. CONCLUSION: Betaine has negative effect on post thaw semen parameters and raffinose at 10 mM concentration improves the fertility from cryopreserved semen. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Cryoletters |
| Volume No.: | 43 |
| Page Number: | 283 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.54680/fr22510110212 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/79092 |
| Appears in Collections: | AS-DPR-Publication |
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