KRISHI
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/80377
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kaur,P | en_US |
dc.contributor.author | Filia,G | en_US |
dc.contributor.author | Singh,S.V | en_US |
dc.contributor.author | Patil,P.K | en_US |
dc.contributor.author | Ravi Kumar,G.V.P.P.S. | en_US |
dc.contributor.author | Sandhu,K.S. | en_US |
dc.date.accessioned | 2023-08-01T09:28:44Z | - |
dc.date.available | 2023-08-01T09:28:44Z | - |
dc.date.issued | 2010-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/80377 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Johne’s disease is chronic granulomatous infectious enteritis of animals caused by Mycobac terium avium subspecies paratuberculosis. A total of 153 animals from 19 dairy farms, 2 gaushalas (unproductive-animal rehabilitation centers), 2 goat and 2 sheep farms from dif ferent districts of the Punjab region were selected on the basis of clinical signs of disease. All samples from cattle (n = 86), buffalo (n = 34), goat (n = 25) and sheep (n = 26) were subjected to Ziehl–Neelsen staining and DNA extraction by a freeze and thaw method. Ziehl–Neelsen staining detected 71% samples positive for acid-fast bacilli whereas IS900 PCR detected 55% positive for Map DNA. IS1311 PCR-REA analysis of IS900 positive samples revealed ‘Bison’ type as the most prevalent (82%) genotype of Map, infecting all domestic ruminants. ‘Cattle’ type was present in a minority of cases (15%) from cattle, buffaloes and goats. This is the first report of ‘Cattle’ type Map from buffalo and goat species in India | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Johne’s disease | en_US |
dc.subject | Mycobacterium avium subspecies | en_US |
dc.subject | paratuberculosis | en_US |
dc.subject | Ziehl–Neelsen staining | en_US |
dc.subject | PCR | en_US |
dc.subject | IS900 | en_US |
dc.subject | IS1311 | en_US |
dc.subject | REA | en_US |
dc.subject | Molecular epidemiology | en_US |
dc.title | Molecular epidemiology of Mycobactetrium avium subspecies paratuberculosis: IS900 PCR identification and IS1311 polymorphism analysis from ruminants in the Punjab region of India. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Not Available | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Central Institute of Brackishwater Aquaculture | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | FS-CIBA-Publication |
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