KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/82750
Title: | Designing of siRNAs for various target genes of Cucumber mosaic virus subgroup IB |
Other Titles: | Not Available |
Authors: | Revathy K A & Bhat A I |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR :: Indian Institute of Spices Research |
Published/ Complete Date: | 2019-01-01 |
Project Code: | Not Available |
Keywords: | Cucumber mosaic virus siRNA designing RNA interference black pepper hairpin construct |
Publisher: | Not Available |
Citation: | Revathy K A & Bhat A I 2019 Designing of siRNAs for various target genes of Cucumber mosaic virus subgroup IB. Indian Journal of Biotechnology 18: 119-125 |
Series/Report no.: | Not Available; |
Abstract/Description: | Cucumber mosaic virus (CMV) is a major production constraint in black pepper causing stunted disease. Resistant varieties are unavailable and control measures are not effective till now. Recently, RNA interference (RNAi) is the most promising strategy for combating virus infection in plants but the effectiveness depends on sequence specificity between the transgene and the targeting virus. This study was undertaken to design the most suitable region for double stranded RNA synthesis with maximum specificity and minimized off–targets for all the five genes of CMV from black pepper. A 400 bp off–target minimized region identified from each of the five genes was subjected to sequence polymorphism study with selected CMV subgroup IB strains and common ‘siRNAs’ were designed in silico. As 3b gene had the least variations (of 17%) with four common and potential siRNAs designed from this region in silico, a hairpin construct was assembled using this region in Agrobacterium that can be used for developing black pepper resistant to selected CMV subgroup IB strains. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Indian Journal of Biotechnology |
Journal Type: | Not Available |
NAAS Rating: | Not Available |
Impact Factor: | Not Available |
Volume No.: | 18 |
Page Number: | 119-125 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/82750 |
Appears in Collections: | HS-IISR-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.