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http://krishi.icar.gov.in/jspui/handle/123456789/9566
Title: | Phylogenetic analysis of glycoprotein B gene sequences of bovine herpesvirus 1 isolates from India reveals the predominance of subtype 1.1. |
Other Titles: | Not Available |
Authors: | Patil SS Prajapati A Hemadri D Suresh KP Desai GS Reddy GBM Chandranaik BM Ranganatha S Rahman H |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics Institute of Animal Health and Veterinary Biologicals, Bengaluru Indian Council of Agricultural Research, New Delhi |
Published/ Complete Date: | 2016-12-01 |
Project Code: | Not Available |
Keywords: | bovine herpesvirus 1-1 glycoprotein B India infectious bovine rhinotracheitis phylogenetic analysis sequencing subtype 1.1 |
Publisher: | Veterinary World |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Aim: This study was conducted for the isolation and molecular characterization of bovine herpesvirus 1 (BoHV-1) isolated from the nasal and vaginal swabs collected from naturally infected cattle showing clinical symptoms of the respiratory disease. Materials and Methods: Isolation of BoHV-1 virus performed on clinical samples collected from 65 cattle from five states of India. The BoHV-1 isolates were further confirmed by polymerase chain reaction (PCR) using primers specific for glycoprotein B (gB) genomic region. PCR amplification was performed using previously published gB gene-specific primer pairs. gB PCR amplicons obtained from all isolates were sequenced, and phylogenetic analysis was performed using software. Results: A total of 12 samples were found positive in cell culture isolation. 11 isolates showed the visible cytopathic effect on Madin-Darby bovine kidney after 72 h. Partial sequence analysis of gB gene of all isolates revealed 99.0-100% homology between them. All isolates showed 99.2-99.8% homology with Cooper stain. Conclusion: BoHV-1.1 is the predominant circulating subtype of BoHV in India, and all isolates have homology with Cooper stain. |
Description: | Not Available |
ISSN: | 2307-8316 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Veterinary World |
NAAS Rating: | Not Available |
Volume No.: | 9(12) |
Page Number: | 1364-1369 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.14202/vetworld.2016.1364-1369 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9566 |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
File | Description | Size | Format | |
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VetWorld-9-1364.pdf | 1.55 MB | Adobe PDF | View/Open |
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