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Enhanced production of pediocin C20 by a native strain of Pediococcus acidilactici C20 in an optimized food-grade medium

IR@CSIR-CFTRI

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Relation http://ir.cftri.com/1481/
PC_38_05
 
Title Enhanced production of pediocin C20 by a native strain of
Pediococcus acidilactici C20 in an optimized
food-grade medium
 
Creator Halami, Prakash M.
Chandrashekar, A.
 
Subject 05 Processing and Engineering
04 Microbiology
 
Description The potentiality of a native isolate, Pediococcus acidilactici C20, to produce quantifiable amounts of pediocin C20 on whey permeate was studied. The molecular basis for the presence of a b-gal like gene was shown by DNA dot-blot technique followed by b-galactosidase (b-Gal)
assay on native polyacrylamide gel as experimental evidence for lactose hydrolysis. Production of pediocin C20 was found to be 1–1.5-fold excess in lactose-based medium as compared to medium with glucose. Optimized whey permeate supplemented with 2% yeast extract gave
cell growth of 3.5, OD600 and pediocin C20 activity of 150 103 AU ml�1 equivalent to that obtained by growth in commercial MRS broth.
 
Date 2005
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://ir.cftri.com/1481/1/Process_Biochemistry_40_%282005%29_1835-1840.pdf
Halami, Prakash M. and Chandrashekar, A. (2005) Enhanced production of pediocin C20 by a native strain of Pediococcus acidilactici C20 in an optimized food-grade medium. Process Biochemistry, 40. pp. 1835-1840.