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Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]

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Relation http://oar.icrisat.org/5113/
http://dx.doi.org/10.1007/s002990050693
 
Title Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]
 
Creator Sairam, R V
Seetharama, N
Devi, P S
Verma, A
Murthy, U R
 
Subject Sorghum
 
Description A protocol for plant regeneration from
mesophyll/protoplasts of sorghum [Sorghum bicolor
(L.) Moench] was developed. The yield of intact protoplasts,
their subsequent divisions and regeneration
were genotype-dependent. The genotype 296B was
always more responsive than IS 32266. For 296B, the
sixth leaf from 18-day-old plants kept in dark for 2 days
before harvesting was found to be the most suitable
source of viable protoplasts. The first division was
observed 10–12 days after plating, and the second division
after 12–14 days. The maximum plating efficiency
was 4.8% in 296 B, followed by 2.48% in IS 32266.
Microcolonies were visible after 25–30 days, and microcalli
after 60–75 days. Whole plants were obtained after
6–8 weeks of culture of microcalli on MS medium
containing 0.2 mg l–1 kinetin and 2 mg l–1 BAP. The
frequency of regeneration in 296B and IS 32266 was
12.80% and 10.58%, respectively. Ten plants transferred
to pots in the glasshouse established well. The
seeds collected from glasshouse-grown plants were
sown in the field where plants were grown to
maturity.
 
Publisher Springer Verlag
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/5113/1/PlantCellRep18%2812%29972-977.pdf
Sairam, R V and Seetharama, N and Devi, P S and Verma, A and Murthy, U R (1999) Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]. Plant Cell Reports, 18 (12). pp. 972-977. ISSN 0721-7714