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Improved Serological Techniques for the Detection and Identification of Groundnut Viruses

OAR@ICRISAT

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Relation http://oar.icrisat.org/5142/
 
Title Improved Serological Techniques for the Detection and Identification of Groundnut Viruses
 
Creator Nolt, B L
Rajeshwari, R
Bharathan, N
Reddy, D V R
 
Subject Groundnut
 
Description Serology is indispensable for the detection
and identification of plant viruses.
Recently, the highly sensitive enzyme-linked
immunosorbent assay (ELISA) and immunosorbent
electron microscopy (ISEM) techniques
have been developed and may replace
some of the conventional serological methods.
The direct or standard double-antibody
sandwich (DAS) form of ELISA. as first
described by Clark and Adams (1977), has
wide applications in plant virology. An
indirect form of ELISA (I-ELISA) has recently
been developed by Barbara and Clark
(1982). The ISEM procedure developed by
Derrick (1973) combines the specificity of
serology with coventional electron microscopy.
DAS-ELISA, I-ELISA and ISEM have
been adapted for the detection and identification
of several groundnut viruses. This paper
will provide a description of each technique
and its application for the detection and characterization
of viruses occurring on groundnut
in India.
 
Date 1983
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/5142/1/0125.pdf
Nolt, B L and Rajeshwari, R and Bharathan, N and Reddy, D V R (1983) Improved Serological Techniques for the Detection and Identification of Groundnut Viruses. In: Proceedings of the National Seminar on Management of Diseases of Oilseed Crops, 21st - 22nd January 1983, Madurai.