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Procedures and protocols to maintain purity and viability of peanut (groundnut) germplasm

OAR@ICRISAT

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Relation http://oar.icrisat.org/5193/
 
Title Procedures and protocols to maintain purity and viability of peanut (groundnut) germplasm
 
Creator Upadhyaya, H D
Pundir, R P S
Singh, S
Gowda, C L L
 
Subject Groundnut
 
Description Successful genebank operations should ensure that pure and healthy seeds are maintained for utilisation in
crop improvement and conserving for posterity. Maintaining peanut germplasm in the genebank requires
considerable attention. Some protocols followed to maintain purity and viability of peanut germplasm are
outlined in this paper. Some distinct measures required for maintenance of peanut germplasm are: (i) raising
the peanut crop in well drained light soils, (ii) growing a minimum of 160 plants per accession to retain
genetic integrity, (iii) verifi cation of accessions’ identity at various stages of crop growth to remove off-type
and diseased plants, (iv) harvesting crop at optimum maturity to obtain healthy seeds, (v) avoiding exposure
of the freshly harvested pods to strong sunlight (temperature >40°C) to maintain seed viability in storage,
(vi) avoiding damage while picking and shelling of pods manually, (vii) conserving seeds at appropriate
moisture content, in medium term (4°C; 30% RH) at 7% and in long term (-20°C) at 3–5% and (viii) monitoring
of seed stock, viability and health at regular intervals to assess the need for regeneration.
As part of the Australian Centre for International Agricultural Research (ACIAR) project ASEM
2001/055, ICRISAT supplied 46 peanut (groundnut) breeding lines for evaluation in Papua New Guinea
(PNG) environments to identify varieties with superior yield and quality over the local check which could
be introduced for cultivation in PNG. It is critical that the PNG organisations take responsibility for maintaining
genetic purity of the newly introduced peanut germplasm. This paper describes the procedures and
protocols that are followed at ICRISAT for maintaining the genetic purity of peanut germplasm. These lines
can be maintained by following the procedures described in this paper.
 
Date 2006
 
Type Conference or Workshop Item
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/5193/1/Procedures%20and%20protocols%20to%20maintain%20purity%20and%20viability%20of.pdf
Upadhyaya, H D and Pundir, R P S and Singh, S and Gowda, C L L (2006) Procedures and protocols to maintain purity and viability of peanut (groundnut) germplasm. In: Improving yield and economic viability of peanut production in Papua New Guinea and Australia: proceedings of a workshop, 18–19 October 2005, Lae, Papua New Guinea.