ICAR Research Data Repository for Knowledge Management
Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/5541/
http://dx.doi.org/10.1016/j.phytochem.2005.04.037 |
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| Title |
Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum
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| Creator |
Gopalakrishnan, S
Beale, M H Ward, J L Strange, R N |
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| Subject |
Chickpea
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| Description |
Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.
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| Publisher |
Elsevier
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| Date |
2005
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/5541/1/Phytochemistry_66_1536%E2%80%931539_2005.pdf
Gopalakrishnan, S and Beale, M H and Ward, J L and Strange, R N (2005) Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum. Phytochemistry, 66 (13). pp. 1536-1539. ISSN 0031-9422 |
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