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Development of a Core RFLP Map in Maize Using an Immortalized F2 Population

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http://www.genetics.org/
 
Title Development of a Core RFLP Map in Maize Using an
Immortalized F2 Population
 
Creator Gardiner, J M
Coe, E H
Melia-Hancock, S
Hoisington, D A
 
Subject Maize
 
Description A map derived from restriction fragment length polymorphisms (RFLPs)i n maize (Zea mays L.) is
presented. The map was constructed in an immortalized Tx303 X C0159 FP mapping population
that allowed for an unlimited number of markers to be mapped and pooled Fs seed to be distributed
to other laboratories. A total of 215 markers consisting of 159 genomic clones, 16 isozymes and 35
cloned geneso f defined function have been placed on 10 chromosomes. An examination of segregation
data has revealed several genomic regions with aberrant segregation ratios favoring either parenot r
the heterozygote. Mapping of cloned genes and isozymes that have been previously mapped by
functional criteria has provided 29 points of alignment with the classical maize genetic map. Screening
of all mapped RFLP probes against a collection of U.S. Corn Belt germplasm using EcoRI, Hind111
and EcoRV has resulted in a set of 97 core markers being defined. The designation of a set of core
markers allows the maize genome to be subdivided into a series of bins which serve as the backbone
for maize genetic information and database boundariesT. he merits and applicationso f core markers v and bins are discussed.
 
Publisher Genetics Society of America
 
Date 1993
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/5713/1/Genetics_134_917-930_1993.pdf
Gardiner, J M and Coe, E H and Melia-Hancock, S and Hoisington, D A (1993) Development of a Core RFLP Map in Maize Using an Immortalized F2 Population. Genetics, 134. pp. 917-930. ISSN 1943-2631