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Effect of 2,4-Dichlorophenoxyacetic Acid and NaCl on the Establishment of Callus and Plant Regeneration in Durum and Bread Wheat

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Relation http://oar.icrisat.org/5734/
http://dx.doi.org/10.1023/B:TICU.0000018389.99656.d8
 
Title Effect of 2,4-Dichlorophenoxyacetic Acid and NaCl on the Establishment of Callus and Plant Regeneration in Durum and Bread Wheat
 
Creator Pellegrineschi, A
Brito, R M
McLean, S
Hoisington, D A
 
Subject Genetics and Genomics
Wheat
 
Description Optimal callus induction and plant regeneration were obtained in bread and durum wheat by manipulating
the NaCl concentration in the induction medium. Immature embryos from a high regeneration line of spring
wheat (Triticum aestivum L.), ‘MPB-Bobwhite 26’, and an elite durum wheat (Triticum turgidum var. durum
L.), ‘Mexicali’, were cultured in E3 induction medium consisting of Murashige and Skoog (MS) medium,
2.5mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D), 2% sucrose and 0.9% Bacto agar. The treated embryos
were transferred to E3 liquid medium supplemented with various levels of 2,4-D and NaCl. Incubation on
medium containing 2.5mg l−1 2,4-D for 45 days produced callus and plant regeneration in ‘MPB-Bobwhite
26’, but lower callus yield and plant regeneration in ‘Mexicali’, indicating that 2,4-D alone was not sufficient
for callus induction and plant regeneration in this durum variety. Callus yield and regeneration frequencies
were higher in ‘Mexicali’ embryos that were incubated in media containing 2mg l−1 2,4-D and 2 mg l−1
NaCl. The presence of NaCl in the medium beyond the initiation phase was detrimental to plant regeneration.
The use o
 
Publisher Kluwer
 
Date 2004
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/5734/1/PCTOCulture_77_%20245%E2%80%93250_2004.pdf
Pellegrineschi, A and Brito, R M and McLean, S and Hoisington, D A (2004) Effect of 2,4-Dichlorophenoxyacetic Acid and NaCl on the Establishment of Callus and Plant Regeneration in Durum and Bread Wheat. Plant Cell, Tissue and Organ Culture, 77 (3). pp. 245-250. ISSN 1573-5044