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Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos

OAR@ICRISAT

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Relation http://oar.icrisat.org/5743/
http://dx.doi.org/10.1007/s001220051255
 
Title Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos
 
Creator Bohorova, N
Zhang, W
Julstrum, P
McLean, S
Luna, M
Brito, R M
Diaz, L
Ramos, M E
Estanol, P
Pacheco, M
Salgado, M
Hoisington, D A
 
Subject Agriculture-Farming, Production, Technology, Economics
Maize
 
Description To enhance the level of resistance to insects in
tropical maize germplasm we have developed techniques
to successfully transform elite tropical maize inbred
based on the activity of specific cryI proteins against
four major maize pests – corn earworm, fall armyworm,
southwestern corn borer and sugarcane borer. Constructs
containing cryIAb or cryIAc synthetic genes were used.
To generate transgenic plants we have established methods
for biolistic bombardment and the selection and regeneration
of immature embryos and calli from the elite
tropical lines CML72, CML216, CML323, CML327 and
hybrids. Transgenic plants resistant to the herbicide BastaTM
contained the bands for the cry, bar and gus genes
as detected by Southern blot analyses. A simple leaf bioassay
presented varying levels of resistance to Southwestern
corn borer of transgenic tropical maize carrying
the cryIAc gene. Analyses of the progenies confirmed
the sexual transmission of the introduced genes and their
stable expression
 
Publisher Springer Verlag
 
Date 1999
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/5743/1/TheorApp%20Gene_99_437%E2%80%93444_1999.pdf
Bohorova, N and Zhang, W and Julstrum, P and McLean, S and Luna, M and Brito, R M and Diaz, L and Ramos, M E and Estanol, P and Pacheco, M and Salgado, M and Hoisington, D A (1999) Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos. TAG Theoretical and Applied Genetics, 99 (3-4). pp. 437-444. ISSN 1432-2242