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Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants

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Title Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants
 
Creator Bohorova, N E
Ginkel, M van
Rajaram, V
Hoisington, D A
 
Subject Wheat
 
Description Six elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, Baviacora
M93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plants
were grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0
mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 medium
supplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plus
different amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rooting
were achieved on basal MS medium with IAA and BAP.
Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media;
each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in the
greenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologically
normal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from Mochis
T88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryos
cultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % of
those grown on E5 medium. These results will form the basis for future efforts aimed at transforming
CIMMYT bread wheat varieties.
IntroductionSix elite CIMMYT bread wheat genotypes Pavon 76, Seri M82, Opata M85, Mochis T88, Baviacora
M93, and the advanced line Attila were evaluated for their response to in vitro tissue culture. Donor plants
were grown at EI Batan and CIANO experiment stations in Mexico in 1992. Immature embryos, about 1.0
mm in length, were excised and placed scutellum side up on callus initiation media. Basal N6 medium
supplemented with dicamba (E1), MS medium containing 2,40 (E3), or MS medium containing 2,40 plus
different amino acids (E5) was used for callus initiation and maintenance. Plant regeneration and rooting
were achieved on basal MS medium with IAA and BAP.
Embryogenic calli with regeneration potentials of 10-92% were obtained on E3 and E5 media;
each embryo produced 5-50 plants. A total of 800 regenerated plants were transferred to pots in the
greenhouse for evaluation and seed production. Of the regenerated plants, 85% were morphologically
normal. reached full maturity, and produced seeds. Seeds (R1) of 360 plants regenerated from Mochis
T88, Baviacora M93, and Altila were planted in the field. Field observations indicated that embryos
cultured on E3 medium resulted in 29% of the progeny producing deviating plants, compared with 21 % of
those grown on E5 medium. These results will form the basis for future efforts aimed at transforming
CIMMYT bread wheat varieties.
Introduction
 
Publisher Adademiai Kiado
 
Date 1995
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/5756/1/CRC_23_3_43-49_1995.pdf
Bohorova, N E and Ginkel, M van and Rajaram, V and Hoisington, D A (1995) Tissue culture response of CIMMYT elite bread wheat cultlvars and evaluation of regenerated plants. Cereal Research Communications, 23 (3). pp. 243-249. ISSN 1788-9170