ICAR Research Data Repository for Knowledge Management
Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/5763/
http://dx.doi.org/10.1007/BF02812266 |
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| Title |
Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme
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| Creator |
Ribaut, J M
Hu, X Hoisington, D A |
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| Subject |
Maize
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| Description |
We describe a new approach for using suitable STS and SSR markers as a powerful molecular tool for screening segregating populations involved in backcross schemes for marker-assisted selection, as a preselection step. Since it can be applied to very large populations, this preselection strategy allows one to increase substantially the pressure of selection at each backcross generation. The technique is fast and reproducible, and can be made even more efficient and costeffective by simultaneous DNA amplification from different primer pairs. In the example illustrated here, three suitable PCR-based markers were used to complete the selection of 300 individuals out of 2300 in less than one month with two people working on the project.
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| Publisher |
Kluwer
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| Date |
1997
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/5763/1/PMBR_15_154-162_1997.pdf
Ribaut, J M and Hu, X and Hoisington, D A (1997) Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme. Plant Molecular Biology Reporter, 15 (2). pp. 154-162. ISSN 1572-9818 |
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