Record Details

A survey of restriction fragment length polymorphisms in tall fescue and its relatives

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/5766/
http://dx.doi.org/10.1139/g91-106
 
Title A survey of restriction fragment length polymorphisms in tall fescue and its relatives
 
Creator Xu, W W
Sleper, D A
Hoisington, D A
 
Subject Genetics and Genomics
 
Description Restriction fragment length polymorphisms (RFLPs) have several advantages over conventional genetic markers and as a result have received increased attention from plant breeders and geneticists. The objective of this study was to construct a tall fescue (Festuca arundinacea Schreb.) genomic library and to survey RFLPs in tall fescue and its relatives. Using plasmid pUC19 as a vector and Escherichia coli XL1-Blue cells as hosts, the first reported PstI genomic DNA library has been established from hexaploid (2n = 6x = 42) tall fescue. The genomic clones were evaluated using nine genotypes from three species of Festuca and three restriction enzymes (BamHI, EcoRI, and HindIII). One hundred and seventy-four probes gave readable results, of which 21% were repetitive and 79% single-copy. The single-copy probes revealed good polymorphism in tall fescue. Approximately 21% of the probes did not cross hybridize to any of the diploids or tetraploids or both and, therefore, represented genome-specific clones
 
Publisher National Research Council Canada
 
Date 1991
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/5766/1/Genome_34_686-692_1991.pdf
Xu, W W and Sleper, D A and Hoisington, D A (1991) A survey of restriction fragment length polymorphisms in tall fescue and its relatives. Genome, 34 (5). pp. 686-692. ISSN 1480-3321