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Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology

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Relation http://oar.icrisat.org/5933/
 
Title Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology
 
Creator Niharika, T
 
Subject Millets
Genetics and Genomics
 
Description Anther culture is an important technique to generate double haploid plants.
Doubled haploids would help to produce homozygous lines in single step
which accelerates the breeding programmes. They are also useful for
studying mutagenesis, dosage effects and interaction & linkage of genes.
Pearl millet is a diploid (2n=14) plant and summer crop that originated
from west Africa. It has become a challenge to develop a reproducible
protocol for double haploids in pearl millet. For culture initiation it is
necessary to select florets and anthers with appropriate developmental
stage of microspore development. The optimal stage for pearl millet anther
culture development has been found to be when the microspores are
uninucleate with the nucleus in the center and when the spike is still
enclosed in the flag leaf sheath. The growth of the donor plants and
developmental stage of microspores have been important factors in the
intiation of anther cultures. The present study aims to assess the effect of
different factors such as growth hormones, media, pretreatments on the
induction of androgenesis (division of microspores) and to develop a
suitable protocol for producing embryoids of pearl millet in vitro using
floret and anther culture.
 
Date 2012
 
Type Thesis
NonPeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/5933/1/Double_haploids_Niharika.pdf
Niharika, T (2012) Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology. Other thesis, Joginpally B.R Engineering College.