Record Details

Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers

OAR@ICRISAT

View Archive Info
 
 
Field Value
 
Relation http://oar.icrisat.org/6109/
http://dx.doi.org/10.4236/ajps.2012.38130
 
Title Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers
 
Creator Datta, S
Gangwar, S
Kumar, Shiv
Gupta, S
Rai, R
Kaashyap, M
Singh, P
Chaturvedi, S K
Singh, B B
Nadarajan, N
 
Subject Genetics and Genomics
Agriculture-Farming, Production, Technology, Economics
 
Description Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.
 
Publisher Scientific Research
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/6109/1/AJPS_3_1085-1091_2012.pdf
Datta, S and Gangwar, S and Kumar, Shiv and Gupta, S and Rai, R and Kaashyap, M and Singh, P and Chaturvedi, S K and Singh, B B and Nadarajan, N (2012) Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers. American Journal of Plant Sciences, 3 (8). pp. 1085-1091. ISSN 2158-2750