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Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers

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Relation http://oar.icrisat.org/6205/
http://www.biomedcentral.com/1471-2229/12/201
 
Title Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers
 
Creator Shutu, X
Dalong, Z
Ye, C
Yi, Z
Shah, T
et al, .
 
Subject Plant Pathology
Maize
 
Description Two F2 populations (K22/CI7 and K22/Dan340, referred to as POP-1 and POP-2, respectively), which share a common parent (K22), were developed and genotyped using a GoldenGate assay containing 1,536 single nucleotide polymorphism (SNP) markers. An integrated genetic linkage map was constructed using 619 SNP markers, spanning a total of 1649.03 cM of the maize genome with an average interval of 2.67 cM. Seventeen quantitative trait loci (QTLs) for all the traits were detected in the first map and 13 in the second. In these two maps, QTLs for different traits were localized to the same genomic regions and some were co-located with candidate genes in the tocopherol biosynthesis pathway. Single QTL was responsible for 3.03% to 52.75% of the phenotypic variation and the QTLs in sum explained23.4% to 66.52% of the total phenotypic variation. A major QTL (qc5-1/qd5-1) affecting alpha-tocopherol (alphaT) was identified on chromosome 5 between the PZA03161.1 and PZA02068.1 in the POP-2. The QTL region was narrowed down from 18.7 Mb to 5.4 Mb by estimating the recombination using high-density markers of the QTL region. This allowed the identification of the candidate gene VTE4 which encodes gamma-tocopherol methyltransferase, an enzyme that transforms gamma-tocopherol (gammaT)to alphaT.
 
Publisher BioMed Central
 
Date 2012
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/6205/1/BMC_Plt_Bio_12_201_2012.pdf
Shutu, X and Dalong, Z and Ye, C and Yi, Z and Shah, T and et al, . (2012) Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers. BMC Plant Biology, 12 (201). pp. 1-28. ISSN 1471-2229