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Methods for the Estimation of Protein in Pigeonpea [Cajanus cajan (L.) Millsp.] and the Relationship Between Whole Grain and Dhal Protein Contents

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Relation http://oar.icrisat.org/6216/
http://dx.doi.org/10.1002/jsfa.2740320711
 
Title Methods for the Estimation of Protein in Pigeonpea
[Cajanus cajan (L.) Millsp.] and the Relationship
Between Whole Grain and Dhal Protein Contents
 
Creator Singh, U
Jambunathan, R
 
Subject Pigeonpea
Food and Nutrition
 
Description Proteindeterrninations of 172 grain and dhal (decorticated dry split seed) pigeonpea
samples were carried out using three methods: (a) rnicrow Kjeldahl (MKJ), (b) colorimetric
estimation of NH4+ with phenol hypochlorite reagents qsing the Technicon
Auto-Analyser (TAA), and (c) dye-binding capacity (DBC) method using Acid Orange
12 dye: Protein perceIltages determined by the TAA and MKJ methods were highly
correlated for whole-grain (0'948**) and dhal (0ยท967**) samples. The DBC method
gave reliable results for dhal samples only. In the DBC procedure, higher protein percentages
were recorded with smaller flour par'tides and longer mixing time, but different
temperatures and durations of heating had no effect. Positive and highly significant
correlations were obtained between the protein values of whole-grain and dhal samples
in all the methods. Small irains gave a lower correlation between whole-grain and
dhal protein content due to the observed negative c;:orrelation between grain size and
percentage of seed~coat.
 
Publisher Society of Chemical Industry
 
Date 1981
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/6216/1/J.Sci.%20Food%20Agric_32_705-710_1981.pdf
Singh, U and Jambunathan, R (1981) Methods for the Estimation of Protein in Pigeonpea [Cajanus cajan (L.) Millsp.] and the Relationship Between Whole Grain and Dhal Protein Contents. Journal of the Science of Food and Agriculture, 32 (7). pp. 705-710. ISSN 1097-0010