Screening Peanut Germ Plasm Lines by Enzyme-Linked Immunosorbent Assay for Seed Transmission of Peanut Mottle Virus
OAR@ICRISAT
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Relation |
http://oar.icrisat.org/6234/
http://www.apsnet.org/PUBLICATIONS/PLANTDISEASE/BACKISSUES/1984/Pages/Number9.aspx |
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Title |
Screening Peanut Germ Plasm Lines by Enzyme-Linked Immunosorbent Assay for Seed Transmission of Peanut Mottle Virus
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Creator |
Bharathan, N
Reddy, D V R Rajeshwari, R Murthy, V K Rao, V R Lister, R M |
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Subject |
Groundnut
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Description |
Enzyme-linked immunosorbent assay (ELISA) was successfully employed for detecting peanut mottle virus (PMV) in peanut seed. Virus could be detected in extracts of infected cotyledons and embryos at dilutions of up to I {3,600, although sensitivity was reduced when infected and healthy seed were mixed. PMV was detected in the testa of about 30% o(infected seed. Complete correlation was observedĀ· among ELISA, in fectivity assays, and growing-out tests for all fourĀ· peanut cultivars tested .. PMV was seed-transmitted in all but two-namely EC 76446 (292) and NC AC 17133 (RF)-of 283 peanut germ plasm lines screened.
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Publisher |
American Phytopathological Society
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Date |
1984
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/6234/1/PlantDisease68n09_757.pdf
Bharathan, N and Reddy, D V R and Rajeshwari, R and Murthy, V K and Rao, V R and Lister, R M (1984) Screening Peanut Germ Plasm Lines by Enzyme-Linked Immunosorbent Assay for Seed Transmission of Peanut Mottle Virus. Plant Disease, 68 (9). pp. 757-758. ISSN 0191-2917 |
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