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Genomic sequence derived simple sequence repeats markers. A case study with medicago spp.

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Relation http://oar.icrisat.org/6285/
http://www.ejbiotechnology.info/content/vol5/issue3/full/2/bip/
 
Title Genomic sequence derived simple sequence repeats markers. A case study with medicago spp.
 
Creator Mahalakshmi, V
Aparna, P
Ramadevi, S
Ortiz, R
 
Subject Genetics and Genomics
 
Description Simple sequence repeats (SSR) or microsatellites are becoming standard DNA markers for plant genome analysis and are being used as markers in marker assisted breeding. De novo generation of microsatellite markers through laboratory-based screening of SSR-enriched genomic libraries is highly time consuming and expensive. An alternative is to screen the public databases of related model species where abundant sequence data is already available. All the genomic sequences of Medicago from the public domain database were searched and analysed for di, tri and tetra nucleotide repeats. Of the total 156 000 sequences which were searched, 7325 sequences contained repeat motif and may yield SSR which will yield product sizes of around 200 bp. Of these, the most abundantly found repeats were the trinucleotide (5210) group. Except for a very small proportion (436), these link to the gene annotation database at TIGR (http://www.tigr.org). To facilitate further exploration of this resource, a dynamic database with options to search and link to other resources is available at (http://www.icrisat.org/text/research/ grep/homepage/genomics/medssrs1.asp) and on CDs from V.Mahalakshmi@cgiar.org
 
Publisher Universidad Católica de Valparaíso - Chile
 
Date 2002
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/6285/1/ElectronicJournalofBiotechnology_5_3_233_242_2002.pdf
Mahalakshmi, V and Aparna, P and Ramadevi, S and Ortiz, R (2002) Genomic sequence derived simple sequence repeats markers. A case study with medicago spp. Electronic Journal of Biotechnology, 5 (3). pp. 233-242. ISSN 0717-3458