ICAR Research Data Repository for Knowledge Management
Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/6467/
http://dx.doi.org/10.1007/s12038-012-9285-4 |
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| Title |
Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton
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| Creator |
Srinivasa Rao, P
Kumar, P S Sonti, R V |
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| Subject |
Genetics and Genomics
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| Description |
Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing
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| Publisher |
Springer
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| Date |
2013
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/6467/1/JournalofBiosciences_38_1_167-172_2013.pdf
Srinivasa Rao, P and Kumar, P S and Sonti, R V (2013) Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton. Journal of Biosciences, 38 (1). pp. 167-172. ISSN 0250-5991 |
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