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Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea

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Relation http://oar.icrisat.org/7276/
http://dx.doi.org/10.3835/plantgenome2013.10.0035
10.3835/plantgenome2013.10.0035
 
Title Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea
 
Creator Varshney, R K
Mohan, S M
Gaur, P M
Chamarthi, S K
Singh, V K
Samineni, S
Swapna, N
Sharma, M
Singh, S
Kaur, L
Pande, S
 
Subject Chickpea
 
Description Fusarium wilt (FW) and Ascochyta blight (AB) are two major
constraints to chickpea (Cicer arietinum L.) production. Therefore,
two parallel marker-assisted backcrossing (MABC) programs
by targeting foc1 locus and two quantitative trait loci (QTL)
regions, ABQTL-I and ABQTL-II, were undertaken to introgress
resistance to FW and AB, respectively, in C 214, an elite cultivar
of chickpea. In the case of FW, foreground selection (FGS) was
conducted with six markers (TR19, TA194, TAA60, GA16, TA110,
and TS82) linked to foc1 in the cross C 214 × WR 315 (FWresistant).
On the other hand, eight markers (TA194, TR58, TS82,
GA16, SCY17, TA130, TA2, and GAA47) linked with ABQTL-I
and ABQTL-II were used in the case of AB by deploying C 214
× ILC 3279 (AB-resistant) cross. Background selection (BGS)
in both crosses was employed with evenly distributed 40 (C
214 × WR 315) to 43 (C 214 × ILC 3279) SSR markers in the
chickpea genome to select plant(s) with higher recurrent parent
genome (RPG) recovery. By using three backcrosses and three
rounds of selfing, 22 BC3F4 lines were generated for C 214 ×
WR 315 cross and 14 MABC lines for C 214 × ILC 3279 cross.
Phenotyping of these lines has identified three resistant lines (with
92.7–95.2% RPG) to race 1 of FW, and seven resistant lines
(with 81.7–85.40% RPG) to AB that may be tested for yield and
other agronomic traits under multilocation trials for possible release
and cultivation.
 
Publisher Crop Science Society of America
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/7276/7/TPG_7_1-11_2014.pdf
Varshney, R K and Mohan, S M and Gaur, P M and Chamarthi, S K and Singh, V K and Samineni, S and Swapna, N and Sharma, M and Singh, S and Kaur, L and Pande, S (2014) Marker-Assisted Backcrossing to Introgress Resistance to Fusarium Wilt Race 1 and Ascochyta Blight in C 214, an Elite Cultivar of Chickpea. The Plant Genome, 7. pp. 1-11. ISSN 1940-3372