Isolation and determination of adenosine 3',5'-cyclic monophosphate from plant tissues
OAR@ICRISAT
View Archive InfoField | Value | |
Relation |
http://oar.icrisat.org/7403/
|
|
Title |
Isolation and determination of adenosine 3',5'-cyclic monophosphate from plant tissues
|
|
Creator |
Narayanan, A
Pradet, A |
|
Subject |
Plant Pathology
|
|
Description |
A procedure has been described to determine picomole levels of adenosine3',5'-cyclic monophosphate (CAMP) in plant materials. The purification of CAMP from other interfering nucleotide and phenolic compounds is made using paper and column chromatography. The resulting CAMP is enzymatically transformed to ATP and estimated by its luminescent reaction with iuciferase. The advantages of this method have been discussed in the light of purification and assay of CAMP. The content of CAMP in lettuce, barley seeds, cabbage leaves, and cultivated mushrooms has been estimated by this procedure |
|
Publisher |
National Institute of Science Communication and Information Resources
|
|
Date |
1974
|
|
Type |
Article
PeerReviewed |
|
Format |
application/pdf
|
|
Language |
en
|
|
Rights |
—
|
|
Identifier |
http://oar.icrisat.org/7403/1/IJEB_12_3_287-289_1974.pdf
Narayanan, A and Pradet, A (1974) Isolation and determination of adenosine 3',5'-cyclic monophosphate from plant tissues. Indian Journal of Experimental Biology, 12 (3). pp. 287-289. ISSN 0019-5189 |
|