Record Details

Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047

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Relation http://oar.icrisat.org/7546/
http://www.iisc.ernet.in/~currsci/aug102006/344.pdf
 
Title Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047
 
Creator Anjaiah, V
Gabriel, D W
 
Subject Plant Pathology
Plant Virology
Agriculture-Farming, Production, Technology, Economics
 
Description Xylella fastidiosa (Xf ) is a fastidious, xylem-inhabiting,
Gram-negative bacterium that causes serious plant
diseases in a wide range of plant species. The most serious
diseases are Pierce’s Disease (PD) of grape and Citrus
Variegated Chlorosis (CVC). Functional genomic analyses
of Xf have been severely limited by lack of a stable
replicative shuttle vector. Plamsid pUFR047, small,
stable, wide host range, conjugative and repW shuttle
vector have been successfully transferred into Xf strains
by electroporation. The vector replicated in a stable
manner for over thirty generations of growth in the
absence of antibiotic selection in Xf strains.
 
Publisher Indian Academy of Sciences
 
Date 2006
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/7546/1/CurrentSc_91_3_344-347_2006.pdf
Anjaiah, V and Gabriel, D W (2006) Stable transformation of Xylella fastidiosa with small repW shuttle vector pUFR047. Current Science, 91 (3). pp. 344-347. ISSN 0011-3891