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The association between exposure to aflatoxin, mutation in TP53, infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India

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Relation http://oar.icrisat.org/7651/
http://dx.doi.org/doi:10.1016/j.mrgentox.2013.12.011
 
Title The association between exposure to aflatoxin, mutation in TP53, infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India
 
Creator Anitha, S
Raghunadharao, D
Waliyar, F
Sudini, H
Parveen, M
Rao, R
Lava Kumar, P
 
Subject Genetics and Genomics
Food and Nutrition
Fertilizer Appications
 
Description Aflatoxin B1 is a carcinogen produced by Aspergillus flavus and a few related fungi that are often present in many food substances. It interacts synergistically with Hepatitis B or C virus (HBV, HBC) infection, thereby increasing the risk of hepatocellular carcinoma (HCC). The G to T transversion at the third position of codon 249 (AGG) of the TP53 gene, substituting arginine to serine, is the most common aflatoxin-induced mutation linked to HCC. This study examined mutations in TP53 by PCR-RFLP analysis and by measurement of an aflatoxin-albumin adduct as a biomarker for human exposure of aflatoxin B1 by indirect-competitive ELISA, in samples collected from healthy controls as well as patients with hepatitis in Hyderabad, Andhra Pradesh, India. A total of 238 blood samples were analyzed the presence of the G to T mutation. Eighteen of these samples were from HBV-positive subjects, 112 of these were from subjects who had HBV-induced liver cirrhosis, and 108 samples were taken from subjects without HBV infection or liver cirrhosis (control group). The G to T mutation was detected in 10 samples, 8 of which were from subjects positive to both HBV and aflatoxin-albumin adduct in blood (p = 0.07); whilst two were from individuals who were HBV-negative, but positive for the aflatoxin-albumin adduct (p = 0.14). The aflatoxin-albumin adduct was detected in 37 of 238 samples, 29 samples were from HBV-positive subjects and eight were from individuals who were positive for both HBV and the TP53 mutation (p = 0.07). The concentration of aflatoxin-albumin adduct ranged from 2.5 to 667 pg/mg albumin. Despite low incidence of the G to T mutation, its detection in subjects positive to aflatoxin-adducts is indicative of a strong association between the mutation and aflatoxin exposure in India.
 
Publisher Elsevier
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
application/pdf
 
Language en
en
 
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Identifier http://oar.icrisat.org/7651/1/mutation-res_am_2014.pdf
http://oar.icrisat.org/7651/7/AFB1%20mutations%20in%20Indian%20population%20%282014%29.pdf
Anitha, S and Raghunadharao, D and Waliyar, F and Sudini, H and Parveen, M and Rao, R and Lava Kumar, P (2014) The association between exposure to aflatoxin, mutation in TP53, infection with hepatitis B virus, and occurrence of liver disease in a selected population in Hyderabad, India. Mutation Research - Genetic Toxicology and Environmental Mutagenesis. pp. 1-20. ISSN 1383-5718