ICAR Research Data Repository for Knowledge Management
The occurrence of maize mosaic virus on sorghum in India
OAR@ICRISAT
View Archive Info| Field | Value | |
| Relation |
http://oar.icrisat.org/7748/
http://dx.doi.org/10.1111/j.1744-7348.1989.tb02106.x |
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| Title |
The occurrence of maize mosaic virus on sorghum in India
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| Creator |
Nigam, S N
Harikrishnan, R Manohar, S K Reddy, D V R Ratna, A S King, S B Bandyopadhyay, R |
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| Subject |
Sorghum
Entomology |
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| Description |
A leaf disease of sorghum (Sorghum bicolor) characterised by fine discontinuous chlorotic streaks between the veins, was observed on sorghum grown during the 1987/88 post-rainy season in peninsular India. Early-infected plants were stunted, had shortened internodes, and produced poorly developed panicles. The virus was transmitted by the delphacid planthopper, Peregrinus maidis. Negatively stained leaf dip preparations contained bullet-shaped virus particles (208 ± 4.4 × 66 ± 1.0 nm) resembling those of rhabdoviruses. In ultrathin sections, the particles budded through the inner nuclear membrane and were present in the cytoplasm within membrane-bound vesicles that were apparently contiguous with the distended outer nuclear membrane. A method for purifying the virus was developed utilising polyethylene glycol (PEG) precipitation, Celite filtration and sucrose densitygradient centrifugation. An antiserum was produced in rabbits with a titre of 1/2650 in the precipitin ring interphase test. The virus could be detected in infected sorghum leaf tissues using a direct antigen coating form of enzyme-linked immunosorbent assay (DAC-ELISA). In immuno-double diffusion tests, the virus reacted positively with antisera to maize mosaic virus (MMV) from Reunion (MMV-RN) and Hawaii (MMV-HI), but not with antisera to barley yellow striate mosaic (BYSMV), cereal chlorotic mottle (CCMV), and cynodon chlorotic streak (CCSV) viruses. Thus, the virus isolated from sorghum is designated the MMV-S isolate. In DAC-ELISA tests, MMV-S reacted positively with antisera to MMV-R, MMV-HI, MMV-Florida isolate, CCSV, and CCMV, and weakly with antiserum to BYSMV. SDS-polyacrylamide gel electrophoresis revealed four major proteins of relative mass Mr 70 000, 59 000, 32 000 and 28 000. In electro-blot immunoassay, MMV and CCSV antisera detected the G and N proteins. These data suggest that MMV-S should be placed in the sonchus yellow net virus subgroup of plant rhabdoviruses
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| Publisher |
Association of Applied Biologists
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| Date |
1989
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| Type |
Article
PeerReviewed |
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| Format |
application/pdf
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| Language |
en
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| Rights |
—
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| Identifier |
http://oar.icrisat.org/7748/1/AAB_114_301-310_1989.pdf
Nigam, S N and Harikrishnan, R and Manohar, S K and Reddy, D V R and Ratna, A S and King, S B and Bandyopadhyay, R (1989) The occurrence of maize mosaic virus on sorghum in India. Annals of Applied Biology, 114 (2). pp. 301-310. ISSN 0003-4746 |
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