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Phylogenetic diversity of Mesorhizobium in chickpea

OAR@ICRISAT

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Relation http://oar.icrisat.org/7922/
http://www.ias.ac.in/jbiosci/kim975.pdf
 
Title Phylogenetic diversity of Mesorhizobium in chickpea
 
Creator Kim, D H
Kaashyap, M
Rathore, A
Das, R A
Parupalli, S
Upadhyaya, H D
Gopalakrishnan, S
Gaur, P M
Singh, S
Kaur, J
Yasin, M
Varshney, R K
 
Subject Chickpea
Genetics and Genomics
 
Description Crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (Cicer arietinum) as
compared with wild species (C. reticulatum, C. bijugum). To explore impact of domestication on symbiosis, 10
accessions of chickpeas, including 4 accessions of C. arietinum, and 3 accessions of each of C. reticulatum
and C. bijugum species, were selected and DNAs were extracted from their nodules. To distinguish chickpea
symbiont, preliminary sequences analysis was attempted with 9 genes (16S rRNA, atpD, dnaJ, glnA, gyrB, nifH,
nifK, nodD and recA) of which 3 genes (gyrB, nifK and nodD) were selected based on sufficient sequence diversity
for further phylogenetic analysis. Phylogenetic analysis and sequence diversity for 3 genes demonstrated that
sequences from C. reticulatum were more diverse. Nodule occupancy by dominant symbiont also indicated that
C. reticulatum (60%) could have more various symbionts than cultivated chickpea (80%). The study demonstrated
that wild chickpeas (C. reticulatum) could be used for selecting more diverse symbionts in the field conditions and it
implies that chickpea domestication affected symbiosis negatively in addition to reducing genetic diversity.
 
Publisher Indian Academy of Sciences
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/7922/1/kim975.pdf
Kim, D H and Kaashyap, M and Rathore, A and Das, R A and Parupalli, S and Upadhyaya, H D and Gopalakrishnan, S and Gaur, P M and Singh, S and Kaur, J and Yasin, M and Varshney, R K (2014) Phylogenetic diversity of Mesorhizobium in chickpea. Journal of Biosciences, 39 (3). pp. 1-5. ISSN 0250-5991