Phylogenetic diversity of Mesorhizobium in chickpea
OAR@ICRISAT
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Relation |
http://oar.icrisat.org/7922/
http://www.ias.ac.in/jbiosci/kim975.pdf |
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Title |
Phylogenetic diversity of Mesorhizobium in chickpea
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Creator |
Kim, D H
Kaashyap, M Rathore, A Das, R A Parupalli, S Upadhyaya, H D Gopalakrishnan, S Gaur, P M Singh, S Kaur, J Yasin, M Varshney, R K |
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Subject |
Chickpea
Genetics and Genomics |
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Description |
Crop domestication, in general, has reduced genetic diversity in cultivated gene pool of chickpea (Cicer arietinum) as compared with wild species (C. reticulatum, C. bijugum). To explore impact of domestication on symbiosis, 10 accessions of chickpeas, including 4 accessions of C. arietinum, and 3 accessions of each of C. reticulatum and C. bijugum species, were selected and DNAs were extracted from their nodules. To distinguish chickpea symbiont, preliminary sequences analysis was attempted with 9 genes (16S rRNA, atpD, dnaJ, glnA, gyrB, nifH, nifK, nodD and recA) of which 3 genes (gyrB, nifK and nodD) were selected based on sufficient sequence diversity for further phylogenetic analysis. Phylogenetic analysis and sequence diversity for 3 genes demonstrated that sequences from C. reticulatum were more diverse. Nodule occupancy by dominant symbiont also indicated that C. reticulatum (60%) could have more various symbionts than cultivated chickpea (80%). The study demonstrated that wild chickpeas (C. reticulatum) could be used for selecting more diverse symbionts in the field conditions and it implies that chickpea domestication affected symbiosis negatively in addition to reducing genetic diversity. |
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Publisher |
Indian Academy of Sciences
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Date |
2014
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/7922/1/kim975.pdf
Kim, D H and Kaashyap, M and Rathore, A and Das, R A and Parupalli, S and Upadhyaya, H D and Gopalakrishnan, S and Gaur, P M and Singh, S and Kaur, J and Yasin, M and Varshney, R K (2014) Phylogenetic diversity of Mesorhizobium in chickpea. Journal of Biosciences, 39 (3). pp. 1-5. ISSN 0250-5991 |
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