Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut
OAR@ICRISAT
View Archive InfoField | Value | |
Relation |
http://oar.icrisat.org/7935/
http://dx.doi.org/10.1007/s11103-014-0193-x |
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Title |
Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut
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Creator |
Zhu, W
Chen, X Li, H Zhu, F Hong, Y Varshney, R K Liang, X |
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Subject |
Groundnut
Genetics and Genomics |
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Description |
The peanut is a special plant for its aerial flowering but subterranean fructification. The failure of peg penetration into the soil leads to form aerial pod and finally seed abortion. However, the mechanism of seed abortion during aerial pod development remains obscure. Here, a comparative transcriptome analysis between aerial and subterranean pods at different developmental stages was produced using a customized NimbleGen microarray representing 36,158 unigenes. By comparing 4 consecutive time-points, totally 6,203 differentially expressed genes, 4,732 stage-specific expressed genes and 2,401 specific expressed genes only in aerial or subterranean pods were identified in this study. Functional annotation showed their mainly involvement in biosynthesis, metabolism, transcription regulation, transporting, stress response, photosynthesis, signal transduction, cell division, apoptosis, embryonic development, hormone response and light signaling, etc. Emphasis was focused on hormone response, cell apoptosis, embryonic development and light signaling relative genes. These genes might function as potential candidates to provide insights into seed abortion during aerial pod development. Ten candidate genes were validated by Real-time RT-PCR. Additionally, consistent with up-regulation of auxin response relative genes in aerial pods, endogenous IAA content was also significantly increased by HPLC analysis. This study will further provide new molecular insight that auxin and auxin response genes potentially contribute to peanut seed and pod development.
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Publisher |
Springer Netherlands
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Date |
2014
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/7935/1/PMB_2014_85_395-409.pdf
Zhu, W and Chen, X and Li, H and Zhu, F and Hong, Y and Varshney, R K and Liang, X (2014) Comparative transcriptome analysis of aerial and subterranean pods development provides insights into seed abortion in peanut. Plant Molecular Biology, 85 (4-5). pp. 395-409. ISSN 1573-5028 |
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