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Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR

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Relation http://oar.icrisat.org/8141/
http://dx.doi.org/10.1007/s00705-014-2139-7
 
Title Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR
 
Creator Anitha, S
Monyo, E S
Okori, P
 
Subject Groundnut
Plant Virology
 
Description Groundnut rosette disease (GRD) is the most devastating disease of groundnuts in sub-Saharan Africa. The disease is caused by synergistic interactions between viruses and virus-like pathogens: groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and a satellite RNA (satRNA). The multi-pathogenic nature of GRD requires efficient diagnostic systems for plant breeding and pathology work. Currently, TAS-ELISA and RT-PCR are used to detect all three pathogens. This approach is time-consuming, expensive and not easily amenable to high throughput. A multiplex PCR-based approach was developed to detect all three pathogens at once, reducing diagnostics costs and time by two thirds. The technique is highly robust and amenable to high throughput, with sensitivity and specificity values of 88 % and 100 %, respectively. The positive predictive value for the technique is 100 %, and the negative predictive value is 90.6 %.
 
Publisher Springer Verlag
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/8141/1/ArchVirol%202014.pdf
Anitha, S and Monyo, E S and Okori, P (2014) Simultaneous detection of groundnut rosette assistor virus (GRAV), groundnut rosette virus (GRV) and satellite RNA (satRNA) in groundnuts using multiplex RT-PCR. Archives of Virology. pp. 1-4. ISSN 0304-8608