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Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)

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Relation http://oar.icrisat.org/8594/
http://dx.doi.org/10.1016/j.ejbt.2014.12.001
10.1016/j.ejbt.2014.12.001
 
Title Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana)
 
Creator De Villiers, S M
Michael, V N
Manyasa, E O
Saiyiorri, A N
Deshpande, S P
 
Subject Finger Millet
 
Description Genetic diversity of finger millet (Eleusine coracana), a nutritious neglected staple cereal in Africa
and South Asia is largely uncharacterized. This study analysed 82 published SSR markers for finger millet
across 10 diverse accessions to compile an informative set for genetic characterisation. Extensive optimization
compared single samples with bulked leaf or bulked DNA samples for capturing within accession genetic
diversity. The markers were evaluated to determine (1) how efficiently they amplified target loci during
high-throughput genotyping with a generic PCR protocol, (2) ease of scoring PCR products and (3)
polymorphism and ability to discern genetic diversity within the tested finger millet germplasm.
 
Publisher Pontificia Universidad Católica de Valparaíso
 
Date 2015-03
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
Rights —
 
Identifier http://oar.icrisat.org/8594/1/Compilation%20of%20an%20informative%20microsatellite.pdf
De Villiers, S M and Michael, V N and Manyasa, E O and Saiyiorri, A N and Deshpande, S P (2015) Compilation of an informative microsatellite set for genetic characterisation of East African finger millet (Eleusine coracana). Electronic Journal of Biotechnology, 18 (2). pp. 77-82. ISSN 0717-3458