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First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa

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Relation http://oar.icrisat.org/8647/
http://dx.doi.org/10.7243/2050-2389-3-3
 
Title First products of DNA marker-assisted selection in sorghum
released for cultivation by farmers in sub-saharan Africa
 
Creator Mohamed, A
Ali, R
Elhassan, O
Suliman, E
Mugoya, C
Masiga, C W
Elhusien, A
Hash, C T
 
Subject Sorghum
 
Description Striga hermonthica (Del.) Benth. is the major biotic constraint to sorghum production. Its control is difficult
and can only be achieved through integrated management strategies that depend mainly on host plant
resistance and enhanced soil fertility. However, breeding for resistance is hampered by the complexity of
host parasite interactions and lack of reliable screening methods. The invention of molecular markers
has enhanced the effectiveness of breeding for resistance. Five genomic regions (QTLs) with linked
markers associated with Striga resistance were mapped in sorghum variety N13 by [10]. In this study, to
increase the efficiency of marker-assisted selection (MAS), 27 EST-SSR markers in close association with
Striga resistance QTLs were also identified and mapped. Populations of backcross (BC3S4) derived from
N13 (Striga resistant) X three farmer preferred sorghum cultivars: Tabat, Wad Ahmed and AG-8 (Striga
susceptible) were generated. Thirty-one lines (BC3S4) with confirmed Striga field resistance were genotyped
with foreground and background selection makers. Twenty resistant lines, with two or more major QTLs
were selected for regional evaluation. Of these 10 lines were selected and advanced for multi-location
testing, together with Wad Ahmed, Tabat, AG-8, N13, SRN39 and IS9830 as checks. Standard variety
trials were conducted in Striga sick plots over three seasons (2009-2011) in Sudan, Gezira Research Station,
Damazine, Sinnar, and Gedarif. Results revealed that four lines (T1BC3S4, AG6BC3S4, AG2BC3S4 and
W2BC3S4) were Striga resistant and agronomically superior with yields ranging from 180% to 298% higher
relative to their recurrent parents. This Striga resistance coupled with superior attributes of the recurrent
parent (including very high yield potentials, high grain quality and drought tolerance) will provide
adaptation and stability across a wide range of environments. These are the first products of DNA markerassisted selection (MAS) in sorghum released for cultivation by farmers in sub-Saharan Africa.
 
Publisher Herbert Publications Ltd.
 
Date 2014
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/8647/1/JPSMB_3_3_2014.pdf
Mohamed, A and Ali, R and Elhassan, O and Suliman, E and Mugoya, C and Masiga, C W and Elhusien, A and Hash, C T (2014) First products of DNA marker-assisted selection in sorghum released for cultivation by farmers in sub-saharan Africa. Journal of Plant Science & Molecular Breeding, 3 (3). pp. 1-10. ISSN 2050-2389