High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
OAR@ICRISAT
View Archive InfoField | Value | |
Relation |
http://oar.icrisat.org/8659/
http://dx.doi.org/10.1007/s00122-015-2488-y |
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Title |
High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus |
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Creator |
Bayer, P E
Ruperao, P Mason, A S Stiller, J Chan, C K K Hayashi, S Long, Y Meng, J Sutton, T Visendi, P Varshney, R K Batley, J Edwards, D |
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Subject |
Genetics and Genomics
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Description |
The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
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Publisher |
Springer-Verlag Berlin Heidelberg
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Date |
2015
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Type |
Article
PeerReviewed |
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Format |
application/pdf
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Language |
en
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Rights |
—
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Identifier |
http://oar.icrisat.org/8659/1/TAG_128_1039-1047_2015.pdf
Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-1706 |
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