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High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus

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Relation http://oar.icrisat.org/8659/
http://dx.doi.org/10.1007/s00122-015-2488-y
 
Title High‑resolution skim genotyping by sequencing reveals the
distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus
 
Creator Bayer, P E
Ruperao, P
Mason, A S
Stiller, J
Chan, C K K
Hayashi, S
Long, Y
Meng, J
Sutton, T
Visendi, P
Varshney, R K
Batley, J
Edwards, D
 
Subject Genetics and Genomics
 
Description The growth of next-generation DNA sequencing technologies has led to a rapid increase in sequence-based genotyping for applications including diversity assessment, genome structure validation and gene-trait association. We have established a skim-based genotyping by sequencing method for crop plants and applied this approach to genotype-segregating populations of Brassica napus and Cicer arietinum. Comparison of progeny genotypes with those of the parental individuals allowed the identification of crossover and non-crossover (gene conversion) events. Our results identify the positions of recombination events with high resolution, permitting the mapping and frequency assessment of recombination in segregating populations.
 
Publisher Springer-Verlag Berlin Heidelberg
 
Date 2015
 
Type Article
PeerReviewed
 
Format application/pdf
 
Language en
 
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Identifier http://oar.icrisat.org/8659/1/TAG_128_1039-1047_2015.pdf
Bayer, P E and Ruperao, P and Mason, A S and Stiller, J and Chan, C K K and Hayashi, S and Long, Y and Meng, J and Sutton, T and Visendi, P and Varshney, R K and Batley, J and Edwards, D (2015) High‑resolution skim genotyping by sequencing reveals the distribution of crossovers and gene conversions in Cicer arietinum and Brassica napus. Theoretical and Applied Genetics, 128 (6). pp. 1039-1047. ISSN 0040-1706